Introduction and objective:
Pyrethroids are widely used pesticides in agriculture. Growing evidence suggests that they may be harmful for human health. Experimental studies show that pyrethroids can induce seizures and impairments of cognitive and motor functions in animals. Caffeine, a widely used psychoactive substance, may influence these disorders. The aim of the current study was to assess whether co-exposure to pyrethroid (zeta-cypermethrin) and caffeine could affect their neurotoxic potential.

Material and methods:
The study was conducted in adult male Swiss mice. The effects of combined exposure to zeta-cypermethrin and caffeine on pyrethroid-induced convulsions, as well as learning in the passive avoidance test and motor coordination in the rota-rod test, were assessed in animals. In the current study, Fury 100 EW, an insecticide with zeta-cypermethrin as an active ingredient, was used. Substances were administered as single intraperitoneal injections. Caffeine was used at doses affecting the neurobehavioural effects of other pesticides, as previously described.

Zeta-cypermethrin-induced seizures were not influenced by caffeine (40 mg/kg) administration. Pyrethroid, at its 1/5 CD50, did not impair learning or motor coordination in mice. Caffeine did not interact with zeta-cypermethrin in the behavioural tests.

Based on the results obtained, it can be concluded that combined exposure to zeta-cypermethrin and caffeine has no effect on their neurotoxic potential in mice.

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