Wpływ flawanonów cytrusowych na homeostazę redoks komórek narażonych na stres oksydacyjny – badania in vitro
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Department of Food and Nutrition, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec Medical University of Silesia, 8 Jednosci Street, 41-200 Sosnowiec, mPoland Head of Department: dr hab. K. Pawłowska-Góral
Department of Analytical Chemistry, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec Medical University of Silesia, 4 Jagiellonska Street, 41-200 Sosnowiec, Poland. Head of Department: prof. dr hab. A. Pyka-Pająk
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Agnieszka Synowiec-Wojtarowicz   

Department of Food and Nutrition, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec Medical University of Silesia, 8 Jednosci Street, 41-200 Sosnowiec, Poland Head of Department: dr hab. K. Pawłowska-Góral
Med Srod. 2016;19(2):32-39
Flavanones (an important group of antioxidants in citrus fruits) are beneficial for health, which is connected with their anti-inflammatory, anti-atherogenic and anti-carcinogenic properties. The present study was undertaken to investigate whether – and in what way – the presence of flavanones influences the redox homeostasis of fibroblasts and alleviates the effects of oxidative stress.

Material and Methods:
The study was conducted on murine fibroblast cell cultures with the addition of flavanones (hesperidin, hesperetin, naringin, naringenin), exposed to oxidative stress (Fe/Asc). In cell homogenates, the activity of superoxide dismutase (SOD) and glutathione peroxidase (GPx) was measured; in the medium, the concentration of nitric oxide was measured.

Results and conclusion:
Our results demonstrate that the addition of naringenin, hesperetin, naringin and hesperidin has a protective effect on cells subjected to oxidative stress The changes observed are particularly visible in the case of aglycone forms of both compounds. Despite the protective properties against oxidative stress which flavanones display, we determined distrubances in redox homeostasis in comparison to the control culture.

This work was supported by the Medical University of Silesia in Katowice
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