Wpływ pojedynczej dawki paracetamolu i/lub N-acetylocysteiny na szczury przewlekle eksponowane na trichloroetylen. III. Wpływ na wybrane izoformy cytochromu P450 w wątrobie
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Department of Histology, Medical University of Silesia, Katowice. Head of Department: R. Wiaderkiewicz MD, PhD, associated professor
Department of Proteomics, Medical University of Silesia, Sosnowiec. Head of Department: Prof. A. Plewka MD PhD
Department of Toxicology, Medical University, Poznań. Head of Department: Prof. J. Jodynis-Liebert PhD
Corresponding author
Andrzej Plewka   

Department of Proteomics, Medical University of Silesia, ul. Ostrogórska 30 41-200 Sosnowiec tel./fax +48 32 364-14-40 e-mail:
Med Srod. 2012;15(4):24-30
In case of overdose of paracetamol the,ability of hepatic biotransformation is saturated and accumulation,of toxic metabolite – NAPQI takes place. Main,CYP isoforms considered to be responsible for bioactivation,of APAP and promoting the same liver intoxication are,CYP2E1, CYP1A2, CYP3A4 and in animals 2B1/2 isoforms additionally. Purpose of this work was examination of paracetamol influence and/or trichloroethylene on the composition of hepatic cytochrome P450 isoforms.

Material and Methods:
Tests were carried out on rats which were treated with trichloroethylene, paracetamol and/or N-acetylcysteine. In the microsomal fraction content of three isoforms of cytochrome P450 i.e. CYP2E1, CYP2B1/2 and CYP1A2 were determined.

Paracetamol slightly stimulated CYP2B1/2 lowering simultaneously level ofCYP1A2. Trichloroethylene stimulated CYP2B1/2. N-acetylcysteine stimulated all tested P450 isoforms. N-acetylcysteine given together with examinated xenobiotics induced studied P450 isoforms.

N-acetylcysteine demonstrated a protective effect on studied CYP isoforms especially when was given upon termination of xenobiotics exposure.

This work was supported by Ministry of Health grants KBN - 4 P05D 021 15
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