The influence of single application of paracetamol and/or N-acetylcysteine on rats in subchronic exposition to trichloroethylene vapours. II. Effect on hepatic glutathione level
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Department of Histology, Medical University of Silesia, Katowice Head of Department: Ryszard Wiaderkiewicz MD, PhD, associated professor
Department of Proteomics, Medical University of Silesia, Sosnowiec Head of Department: Prof. Andrzej Plewka MD PhD
Department of Toxicology, Medical University, Poznań Head of Department: Prof. Jadwiga Jodynis-Liebert PhD
Corresponding author
Danuta Plewka   

Department of Proteomics Medical University of Silesia, ul. Ostrogórska 30, 41-200 Sosnowiec tel./fax +48 32 364-14-40
Med Srod. 2012;15(3)
Feature of modern existing hazards both environmental and occupational is cumulative exposure often leading to unexpected response of the organism resulting, among other things, in interactions with cytochrome P450 system involved in biotransformation of trichloroethylene and paracetamol. Hepatotoxity of paracetamol is closely connected with hepatic glutathione level. „In therapy of acute paracetamol poisoning application of N-acetylcysteine as a factor, which protects GSH level in cells, is recommended.”

Material and Methods:
Tests were performed on rats which were treated with trichloroethylene, paracetamol and/or N-acetylcysteine. In rat liver total level of glutathione was determined i.e. reduced and oxidized form.

Paracetamol just after completion of the exposure affected the glutathione level. Trichloroethylene throughout the period of observation stimulated growth of glutathione level in liver. N-acetylcysteine didn’t have any influence on the level of investigated tripeptyde.

N-acetylcysteine removes negative effect of paracetamol especially when it’s applied with 2-hour delay. After exposure for trichloethylene immediate application of N-acetylcysteine caused noticeable lowering of glutathione level. Cumulative exposure for three xenobiotics had positive influence for glutathione level in rat liver.

The study was supported by a grant from the State Committee for Scientific Research (KBN – 4 P05D 021 15)
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