Antimicrobial resistance and molecular characteristics of Streptococcus agalactiae isolated from women of reproductive age
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Department of Microbiology, University of Agriculture in Cracow
Centre for Microbiological Research and Autovaccines, Cracow Head of the Department of Microbiology: dr hab. eng. M. Chmiel
Anna Lenart-Boroń   

Department of Microbiology University of Agriculture in Kraków Mickiewicza ave 24/28 phone no.: 126624096
Med Srod. 2016;19(4):27–33
Streptococcus agalactiae infections are among the most significant causes of neonatal invasive diseases. Proper screening and detection of pregnant women carrying GBS allows intrapartum administration of antibiotic prophylaxis and is an effective measure in preventing transmission of bacteria from mother to newborns.

Material and Methods:
Sixty three bacterial strains were isolated from vaginal swabs from pregnant and nonpregnant women of reproductive age. Species were identified by colony morphology, haemolysis type, Gram staining and SLIDEX® Strepto Plus latex test. Antimicrobial resistance of 56 strains was determined using disk-diffusion method. The presence of molecular resistance determinants was assessed using PCR with specific primers, and capsular types were identified using multiplex PCR.

None of the strains were resistant to the first drug of choice, penicillin. A large percentage of isolates (78.6%) were resistant to doxycycline. The prevalence of resistance to macrolides and lincosamides, antibiotics used in women allergic to penicillin, was high. Those results corresponded with PCR tests, as tetM and ermA1 were most frequently detected genes (98.4 and 87.3%, respectively). 7.94% of strains possessed 7 different out of 13 tested genes determining resistance to different groups of antimicrobials. Among the capsular types, Ia, which proved to be associated with the most severe and invasive infections in mothers and neonates, was the most prevalent (65.08%).

Even though they are susceptible to penicillin, multidrug resistance is common among S. agalactiae strains isolated from women of reproductive age and this resistance can be caused by more than one gene per single isolate.

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