Evaluation of the antimicrobial resistance and genetic similarity among Campylobacter Coli and Campylobacter Jejuni strains isolated from clinical materiał and environmental sources
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Katedra i Zakład Higieny i Epidemiologii. Kierownik: dr hab. n. med. Jacek J. Klawe, prof. UMK
Katedra i Zakład Mikrobiologii. Kierownik: dr hab. Eugenia Gospodarek, prof. UMK Collegium Medicum im. L. Rydygiera w Bydgoszczy, Uniwersytet Mikołaja Kopernika w Toruniu
Bernadeta Szczepańska   

Katedra i Zakład Higieny i Epidemiologii Curie-Skłodowskiej 9, 85-094 Bydgoszcz
Med Srod. 2010;13(2):63–69
The aim of the work was to determine susceptibility to drugs and the genetic similarity among the C. jejuni and C. coli strains that were isolated from different sources in 2008–2009.

Material and Methods:
100 Campylobacter strains were analysed: 48 strains were collected from poultry, 20 from children stools, 19 from water samples and 13 from liquid manure. MIC value for erythromycin, azithromycin, ciprofloxacin, and tetracycline were determined with the E-test method. The chromosomal DNA was separated by PFGE, after digestion with restriction enzyme SmaI.

All the analyzed strains were susceptible to macrolides, 52.0% of them to tetracycline (36.0% in 2008 and 68.0% in 2009), and 21.0 % to ciprofloxacin (16.0 % in 2008 and 26.0 % in 2009). The MIC50 value obtained for erythromycin among the analyzed strains was 0.25 mcg/ml and MIC90 0.75 mcg/ml, for azithromycin respectively 0.047 mcg/ml and 0.19 mcg/ml. MIC50 and MIC90 values for ciprofloxacin were 32 mcg/ml and for tetracycline 0.5 and 256 mcg/ml respectively. Among the strains of the same origin identical and similar chromosomal DNA patterns were observed. Particular Campylobacter strains that were isolated from different samples also presented the same chromosomal DNA pattern.

The lowest MIC values were obtained for azithromycin. An increase in the percentage of strains susceptible to ciprofloxacin and tetracycline was observed in 2009 compared to the results obtained in 2008. In a given environment probably identical and closely related strains survive.

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